Influencia de la longitud de onda de luz monocromática en la resonancia del plasmón de nanopartículas de plata biosíntetizadas y su aplicación en la inhibición de bacterias E. coli

Surface plasmon resonance (SPR) is a characteristic present in metallic nanoparticles which are associated with the collective oscillation of free electrons. In this research work, silver nanoparticles (NP Ag) are considered, which have been obtained by the green synthesis method (biosynthesis) using the extract of grape residues from the wine industry as a reducer; These nanoparticles in research play the role of inhibiting agent of E. Coli bacteria. The inhibition efficiency of NP Ag can increase based on the frequency of the incident energy motivated by the wavelength of each monochromatic color, raising the level of oscillation of the surface plasmon, this is how the influence is investigated as a function of seven wavelengths corresponding to the visible spectrum. The results show a correct synthesis of silver nanoparticles obtained by the green route, since the characterization by UV-vis spectrophotometry shows the RPS peak at 422.61 nm typical for this type of nanomaterial; Likewise, the evaluation by scanning electron microscopy indicates a defined spherical geometry with an average size of 32.66 nm; the effect of NP Ag with light influence suggests a high efficiency in the inhibition of E. Coli, specifically when it is under the influence of the Monochromatic blue wavelength (11.2 mm halo inhibition - 69.6% above the sample without influence of light). This inhibiting effect adds to the already known intrinsic properties of silver to inhibit bacterial agents.