Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates

Sol Rodríguez; Francesca Gatto; Luca Pesce; Claudio Canale; Pier Paolo Pompa; Giuseppe Bardi; Daniel Lopez; Fernando G. Torres

doi:10.1080/00914037.2017.1297942

Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates

Sol Rodríguez, Francesca Gatto, Luca Pesce, Claudio Canale, Pier Paolo Pompa, Giuseppe Bardi, Daniel Lopez, Fernando G. Torres

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Exopolysaccharides (EPS) extracted from Nostoc commune cyanobacteria were used to prepare low-adhesion cell substrates for tumor-selective cell growth. The substrates were reinforced with chitin whiskers and starch nanoparticles and characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and tensile tests. Two anchorage independent cells, green fluorescent protein-modified HeLa and THP-1 cells were seeded on the samples to assess their interaction with the composites. The results confirmed the cell–surface interaction of EPS-based composites. The mechanical properties of these films can be tailored by controlling the type and concentration of the reinforcement, which can be used in the design of different types of biomaterials.

Original language	English
Pages (from-to)	333-339
Number of pages	7
Journal	International Journal of Polymeric Materials and Polymeric Biomaterials
Volume	67
Issue number	6
DOIs	https://doi.org/10.1080/00914037.2017.1297942
State	Published - 13 Apr 2018
Externally published	Yes

Keywords

Cell attachment
cell–surface interaction
exopolysaccharides
Nostoc commune

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10.1080/00914037.2017.1297942

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Rodríguez, S., Gatto, F., Pesce, L., Canale, C., Pompa, P. P., Bardi, G., Lopez, D., & Torres, F. G. (2018). Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates. International Journal of Polymeric Materials and Polymeric Biomaterials, 67(6), 333-339. https://doi.org/10.1080/00914037.2017.1297942

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title = "Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates",

abstract = "Exopolysaccharides (EPS) extracted from Nostoc commune cyanobacteria were used to prepare low-adhesion cell substrates for tumor-selective cell growth. The substrates were reinforced with chitin whiskers and starch nanoparticles and characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and tensile tests. Two anchorage independent cells, green fluorescent protein-modified HeLa and THP-1 cells were seeded on the samples to assess their interaction with the composites. The results confirmed the cell–surface interaction of EPS-based composites. The mechanical properties of these films can be tailored by controlling the type and concentration of the reinforcement, which can be used in the design of different types of biomaterials.",

keywords = "Cell attachment, cell–surface interaction, exopolysaccharides, Nostoc commune",

author = "Sol Rodr{\'i}guez and Francesca Gatto and Luca Pesce and Claudio Canale and Pompa, {Pier Paolo} and Giuseppe Bardi and Daniel Lopez and Torres, {Fernando G.}",

note = "Publisher Copyright: {\textcopyright} 2017 Taylor & Francis.",

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day = "13",

doi = "10.1080/00914037.2017.1297942",

language = "English",

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Rodríguez, S, Gatto, F, Pesce, L, Canale, C, Pompa, PP, Bardi, G, Lopez, D & Torres, FG 2018, 'Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates', International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 67, no. 6, pp. 333-339. https://doi.org/10.1080/00914037.2017.1297942

Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates. / Rodríguez, Sol; Gatto, Francesca; Pesce, Luca et al.
In: International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 67, No. 6, 13.04.2018, p. 333-339.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates

AU - Rodríguez, Sol

AU - Gatto, Francesca

AU - Pesce, Luca

AU - Canale, Claudio

AU - Pompa, Pier Paolo

AU - Bardi, Giuseppe

AU - Lopez, Daniel

AU - Torres, Fernando G.

PY - 2018/4/13

Y1 - 2018/4/13

N2 - Exopolysaccharides (EPS) extracted from Nostoc commune cyanobacteria were used to prepare low-adhesion cell substrates for tumor-selective cell growth. The substrates were reinforced with chitin whiskers and starch nanoparticles and characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and tensile tests. Two anchorage independent cells, green fluorescent protein-modified HeLa and THP-1 cells were seeded on the samples to assess their interaction with the composites. The results confirmed the cell–surface interaction of EPS-based composites. The mechanical properties of these films can be tailored by controlling the type and concentration of the reinforcement, which can be used in the design of different types of biomaterials.

AB - Exopolysaccharides (EPS) extracted from Nostoc commune cyanobacteria were used to prepare low-adhesion cell substrates for tumor-selective cell growth. The substrates were reinforced with chitin whiskers and starch nanoparticles and characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and tensile tests. Two anchorage independent cells, green fluorescent protein-modified HeLa and THP-1 cells were seeded on the samples to assess their interaction with the composites. The results confirmed the cell–surface interaction of EPS-based composites. The mechanical properties of these films can be tailored by controlling the type and concentration of the reinforcement, which can be used in the design of different types of biomaterials.

KW - Cell attachment

KW - cell–surface interaction

KW - exopolysaccharides

KW - Nostoc commune

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U2 - 10.1080/00914037.2017.1297942

DO - 10.1080/00914037.2017.1297942

M3 - Article

AN - SCOPUS:85023208625

SN - 0091-4037

VL - 67

SP - 333

EP - 339

JO - International Journal of Polymeric Materials and Polymeric Biomaterials

JF - International Journal of Polymeric Materials and Polymeric Biomaterials

IS - 6

ER -

Monitoring cell substrate interactions in exopolysaccharide-based films reinforced with chitin whiskers and starch nanoparticles used as cell substrates

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Keywords

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