TY - GEN
T1 - Pre-treatments applied to rice husk enzymatic hydrolysis
T2 - 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020
AU - Aredo, Fernando
AU - Rojas, Meliza Lindsay
AU - Pagador, Sandra
AU - Lescano, Leslie
AU - Sanchez-Gonzalez, Jesús
AU - Linares, Guillermo
N1 - Publisher Copyright:
© 2020 Latin American and Caribbean Consortium of Engineering Institutions. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Freezing (-20 °C, 12h) and alkaline (NaOH 8%) pretreatments were applied individually and combined in rice husk (Oryza sativa L.) before their hydrolysis with cellulase (EC: 3.2.1.4.). The effects on structural modifications, lignin content, cellulose, hemicellulose content and glucose production were evaluated. In addition, the glucose production kinetics were described by using the Peleg model. The homogenous rice husk (1 g) with and without pretreatments was hydrolysed with 150 U of Cellulase in 10 ml of acetate for 60 h (37 °C, pH 5.5, 100 rpm). As results, the SEM images evidenced porous microstructures with less agglomeration generated by all pre-treatments, which were intensified by the combined pretreatment. This pre-treatment allowed to obtain higher cellulose (62.51 ± 0.3 %) content. Besides, the glucose content after pretreatments increased. The Peleg model parameters from glucose production kinetics during enzymatic hydrolysis were related to initial glucose content (G0), glucose production rate (1/k1) and maximum glucose yield (1/k2). After enzymatic hydrolysis process, compared to control glucose yield (0.359 ± 0.002 g G/g rice husk), this was 27%, 71% and 88% higher for freezing, alkaline and combined pre-treatments respectively.
AB - Freezing (-20 °C, 12h) and alkaline (NaOH 8%) pretreatments were applied individually and combined in rice husk (Oryza sativa L.) before their hydrolysis with cellulase (EC: 3.2.1.4.). The effects on structural modifications, lignin content, cellulose, hemicellulose content and glucose production were evaluated. In addition, the glucose production kinetics were described by using the Peleg model. The homogenous rice husk (1 g) with and without pretreatments was hydrolysed with 150 U of Cellulase in 10 ml of acetate for 60 h (37 °C, pH 5.5, 100 rpm). As results, the SEM images evidenced porous microstructures with less agglomeration generated by all pre-treatments, which were intensified by the combined pretreatment. This pre-treatment allowed to obtain higher cellulose (62.51 ± 0.3 %) content. Besides, the glucose content after pretreatments increased. The Peleg model parameters from glucose production kinetics during enzymatic hydrolysis were related to initial glucose content (G0), glucose production rate (1/k1) and maximum glucose yield (1/k2). After enzymatic hydrolysis process, compared to control glucose yield (0.359 ± 0.002 g G/g rice husk), this was 27%, 71% and 88% higher for freezing, alkaline and combined pre-treatments respectively.
KW - Alkaline pre-treatment
KW - Cellulase
KW - Enzymatic hydrolysis
KW - Freezing
KW - Rice husk
UR - http://www.scopus.com/inward/record.url?scp=85096762326&partnerID=8YFLogxK
U2 - 10.18687/LACCEI2020.1.1.42
DO - 10.18687/LACCEI2020.1.1.42
M3 - Conference contribution
AN - SCOPUS:85096762326
T3 - Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology
BT - 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology
Y2 - 27 July 2020 through 31 July 2020
ER -