Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman

Jimy Oblitas; Jordana Morales; Fanny Rimarachin; Wilson Castro; Tony Chuquizuta

doi:10.18687/LACCEI2020.1.1.219

Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman

Translated title of the contribution: Selection of key factors in the performance of Kluyveromyces fragilis biomass from serum of cheese by using a Placket-Burman design

Jimy Oblitas
, Jordana Morales
, Fanny Rimarachin
, Wilson Castro
, Tony Chuquizuta

Universidad Nacional de Cajamarca
Universidad Privada del Norte
Universidad Nacional de Frontera

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

In this work a study of biomass production from deproteinized serum of cheese was made by using yeast Kluyveromyces fragilis from the Spanish Type Culture Collection with the objective of providing an alternative of use for the subproduct “serum of cheese”, since this product is highly pollutant. Ammonium salts, potassium, magnesium, calcium, iron, zinc and manganese were added to the deproteinized serum obtained by acidification and warming. PH was adjusted to 4.5; the yeast was sterilized and inoculated. The maximum rate of growing for the yeast was of 0.0398 h-1 and the coefficient of biomass produced per unit of mass of substrate consumed was of 0.272. A model of screening of Placket Burman design with 11 variables was generated, resulting as the most important ones: the deproteinized serum of cheese, yeast extract and ferrous sulfate FeSO4, being the first one the most significant (p<0.05) for the obtaining of Kluyveromyces fragilis biomass.

Translated title of the contribution	Selection of key factors in the performance of Kluyveromyces fragilis biomass from serum of cheese by using a Placket-Burman design
Original language	Spanish
Title of host publication	18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology
Subtitle of host publication	"Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020
ISBN (Electronic)	9789585207141
DOIs	https://doi.org/10.18687/LACCEI2020.1.1.219
State	Published - 2020
Event	18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020 - Virtual, Online Duration: 27 Jul 2020 → 31 Jul 2020

Publication series

Name	Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology
ISSN (Electronic)	2414-6390

Conference

Conference	18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020
City	Virtual, Online
Period	27/07/20 → 31/07/20

Access to Document

10.18687/LACCEI2020.1.1.219

Cite this

Oblitas, J., Morales, J., Rimarachin, F., Castro, W., & Chuquizuta, T. (2020). Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman. In 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020 (Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology). https://doi.org/10.18687/LACCEI2020.1.1.219

Oblitas, Jimy ; Morales, Jordana ; Rimarachin, Fanny et al. / Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman. 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020. 2020. (Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology).

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title = "Selecci{\'o}n de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un dise{\~n}o Placket-Burman",

abstract = "In this work a study of biomass production from deproteinized serum of cheese was made by using yeast Kluyveromyces fragilis from the Spanish Type Culture Collection with the objective of providing an alternative of use for the subproduct “serum of cheese”, since this product is highly pollutant. Ammonium salts, potassium, magnesium, calcium, iron, zinc and manganese were added to the deproteinized serum obtained by acidification and warming. PH was adjusted to 4.5; the yeast was sterilized and inoculated. The maximum rate of growing for the yeast was of 0.0398 h-1 and the coefficient of biomass produced per unit of mass of substrate consumed was of 0.272. A model of screening of Placket Burman design with 11 variables was generated, resulting as the most important ones: the deproteinized serum of cheese, yeast extract and ferrous sulfate FeSO4, being the first one the most significant (p<0.05) for the obtaining of Kluyveromyces fragilis biomass.",

keywords = "Biomass, Cheese whey, Kluyveromyces fragilis, Placket Burman, Screening",

author = "Jimy Oblitas and Jordana Morales and Fanny Rimarachin and Wilson Castro and Tony Chuquizuta",

note = "Publisher Copyright: {\textcopyright} 2020 Latin American and Caribbean Consortium of Engineering Institutions. All rights reserved.; 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: {"}Engineering, Integration, and Alliances for a Sustainable Development{"} {"}Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy{"}, LACCEI 2020 ; Conference date: 27-07-2020 Through 31-07-2020",

year = "2020",

doi = "10.18687/LACCEI2020.1.1.219",

language = "Espa{\~n}ol",

series = "Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology",

booktitle = "18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology",

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Oblitas, J, Morales, J, Rimarachin, F, Castro, W & Chuquizuta, T 2020, Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman. in 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020. Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology, 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020, Virtual, Online, 27/07/20. https://doi.org/10.18687/LACCEI2020.1.1.219

Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman. / Oblitas, Jimy; Morales, Jordana; Rimarachin, Fanny et al.
18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020. 2020. (Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman

AU - Oblitas, Jimy

AU - Morales, Jordana

AU - Rimarachin, Fanny

AU - Castro, Wilson

AU - Chuquizuta, Tony

PY - 2020

Y1 - 2020

N2 - In this work a study of biomass production from deproteinized serum of cheese was made by using yeast Kluyveromyces fragilis from the Spanish Type Culture Collection with the objective of providing an alternative of use for the subproduct “serum of cheese”, since this product is highly pollutant. Ammonium salts, potassium, magnesium, calcium, iron, zinc and manganese were added to the deproteinized serum obtained by acidification and warming. PH was adjusted to 4.5; the yeast was sterilized and inoculated. The maximum rate of growing for the yeast was of 0.0398 h-1 and the coefficient of biomass produced per unit of mass of substrate consumed was of 0.272. A model of screening of Placket Burman design with 11 variables was generated, resulting as the most important ones: the deproteinized serum of cheese, yeast extract and ferrous sulfate FeSO4, being the first one the most significant (p<0.05) for the obtaining of Kluyveromyces fragilis biomass.

AB - In this work a study of biomass production from deproteinized serum of cheese was made by using yeast Kluyveromyces fragilis from the Spanish Type Culture Collection with the objective of providing an alternative of use for the subproduct “serum of cheese”, since this product is highly pollutant. Ammonium salts, potassium, magnesium, calcium, iron, zinc and manganese were added to the deproteinized serum obtained by acidification and warming. PH was adjusted to 4.5; the yeast was sterilized and inoculated. The maximum rate of growing for the yeast was of 0.0398 h-1 and the coefficient of biomass produced per unit of mass of substrate consumed was of 0.272. A model of screening of Placket Burman design with 11 variables was generated, resulting as the most important ones: the deproteinized serum of cheese, yeast extract and ferrous sulfate FeSO4, being the first one the most significant (p<0.05) for the obtaining of Kluyveromyces fragilis biomass.

KW - Biomass

KW - Cheese whey

KW - Kluyveromyces fragilis

KW - Placket Burman

KW - Screening

UR - https://www.scopus.com/pages/publications/85096780142

U2 - 10.18687/LACCEI2020.1.1.219

DO - 10.18687/LACCEI2020.1.1.219

M3 - Contribución a la conferencia

AN - SCOPUS:85096780142

T3 - Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology

BT - 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology

T2 - 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020

Y2 - 27 July 2020 through 31 July 2020

ER -

Oblitas J, Morales J, Rimarachin F, Castro W, Chuquizuta T. Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman. In 18th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology: "Engineering, Integration, and Alliances for a Sustainable Development" "Hemispheric Cooperation for Competitiveness and Prosperity on a Knowledge-Based Economy", LACCEI 2020. 2020. (Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology). doi: 10.18687/LACCEI2020.1.1.219

Selección de factores clave en el rendimiento de biomasa de Kluyveromyces fragilis a partir de suero de queso usando un diseño Placket-Burman

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